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My Lab Essentials: Dr Tom Brown (Jr)

SelectScience
19 Nov 2014
Kathryn Rix
Administrator / Office Personnel

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Whether it makes our lab work easier, helps to speed up our research or just saves us from doing a laborious task, we all have lab equipment we just can’t do without. In this first article in a new series, My Lab Essentials, Dr Tom Brown (Jr), Director and Head of Oxford R&D Laboratory at ATDBio, discusses the research and development his laboratory carries out to produce highly modified DNA oligonucleotides, and the lab equipment that helps his team to do it.

Tom says: “The oligonucleotides produced by ATDBio are unique and of the highest quality, and are used in a range of research areas including genetics, genomics, biochemistry, molecular biology and nanotechnology. We have recently moved to larger, custom built laboratories, which has allowed us to invest in new equipment and staff to meet the demand from our customers.

“The research carried out by ATDBio is successful because of our talented staff, and is made easier by the innovative equipment that we use.”

Here are Tom’s lab essentials:

1. Xevo G2 QTof with ACQUITY UPLC H-Class System from Waters

Combining the Xevo G2 QTof with the ACQUITY UPLC H-Class System enables good separation with short run times, and allows us to obtain accurate MS data on DNA oligonucleotides. The high sensitivity of the Xevo G2 QTof allows us to interrogate impurities arising from oligonucleotide synthesis; this information allows us to optimize DNA synthesis and ultimately to produce better quality oligonucleotides.

2. ShimadzuProminence LC-20AP Preparative HPLC system

Powerful, good value, easy to use prep. HPLC system with intuitive software.

3. ThermoMixer® C from Eppendorf

These versatile temperature-controlled mixers are useful for all kinds of chemical reactions in different vessels. We use them extensively for DNA labelling and enzyme reactions.

4. CLARIOstar multi-mode microplate reader from BMG LABTECH

Microplate reader with monochromator, spectrometer, and filters. We make DNA with many different fluorescent dyes, and the monochromator allows us to measure fluorescence intensity at different emission/absorbance wavelengths without needing a separate filter for each dye. Can also be measure emission and absorbance spectra, and for routine UV measurements.

5. OligoPilot™ 400 from GE Healthcare

An automated system for the large-scale synthesis of DNA and RNA oligonucleotides.

Which lab equipment makes your research possible? Review them here.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Lab EssentialsLab essentials include laboratory consumables that are vital to the operation of all laboratories.

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