Listeria species PCR Assay Offers Validated Method for Fast, Simple Detection of Food Pathogens

26 Aug 2013

Product news

Food microbiology laboratories can now confidently test to a recognized standard for Listeria species with the Thermo Scientific SureTect Listeria species Assay, which has been granted Performance Tested Methods status by the AOAC Research Institute.

Listeria species originate from the environment and can be commonly found in soils and untreated water, resulting in a risk for animals and plants destined for human consumption.

The SureTect Listeria species PCR Assay has proven to be comparable to the ISO 11290-1 reference method. ISO methods are chosen by many companies against which to test human food, animal feed and environmental samples. The assay, as well as the entire suite of SureTect products, will be on display at the Thermo Scientific booth (208) during the AOAC Annual Meeting and Exposition at the Palmer House Hilton in Chicago, August 25-28.

“We are delighted to have received validation of our Listeria species PCR assay, which is one of a number of assays we offer for molecular food testing,” says Sumi Thaker, vice president of global marketing, microbiology, for Thermo Fisher Scientific. “The SureTect Real-Time PCR System has been designed to streamline test workflow and reduce time to result, which are primary considerations in today’s pathogen test selection, and all of our current assays are now validated according to the AOAC-RI Performance Tested Methods program.”

The SureTect System was developed to detect microorganisms quickly and accurately in a broad range of foods and associated samples. This unique solution combines:

• Ease of use: Pre-filled lysis tubes and tableted PCR reagents to minimize the number of pipetting steps and hands-on time
• Speed: Optimized single enrichment step for key food matrices and straightforward sample lysis in less than 20 minutes
• Convenience: Common PCR protocols facilitate efficient processing of multiple assays in the same run
• Superior PCR Technology: Probe-based real-time assays for unparallel sensitivity and specificity

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.AOAC MethodsFood SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.ListeriaReal-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.
Listeria species PCR Assay Offers Validated Method for Fast, Simple Detection of Food Pathogens