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HPLC Column Introduced to Simplify and Improve Separations of Herbicides Diquat and Paraquat

Thermo Fisher Scientific
8 Oct 2012

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Thermo Fisher Scientific Inc., the world leader in serving science, today introduced the Thermo Scientific Acclaim Trinity Q1 HPLC column, designed to facilitate high-resolution and high-throughput trace analysis of the herbicides diquat and paraquat in plants by liquid chromatography-mass spectrometry (LC-MS).

According to the U.S. Environmental Protection Agency Healthcare Handbook, diquat and paraquat are non-selective herbicides that are widely-used in agriculture and by government agencies and industries for control of weeds. Both are considered to be toxic, and a recent study suggested a linkage between paraquat and Parkinson’s Disease among farm workers. While the dangers of the pesticides, when concentrated, are well documented, the effects of residual levels of these pesticides in food require additional research.

Acclaim™ Trinity™ Q1 columns have demonstrated the ability to deliver the retention, resolution, peak shape and speed needed to improve diquat and paraquat LC-MS analysis in common terrestrial and aquatic plant matrices. The stable baseline separation for diquat and paraquat reduces the challenges of achieving accurate detection and quantitation compared to reversed-phase column methods.

The new column contains innovative Nanopolymer Silica Hybrid (NSH) technology that, unlike other support systems, does not require ion pair reagents or exotic mobile phases to separate diquat and paraquat for LC-MS analysis. The cation-exchange function provides retention and separation for diquat and paraquat while the anion-exchange moiety effectively deactivates the undesirable interaction between the surface silanols and the analytes.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity.  Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.PesticidesPesticides are used to protect crops from diseases, pests and unwanted plants. They are classed as persistent organic pollutants (POPs) and can be harmful to humans. The Environmental Protection Agency (EPA) currently regulates the use of pesticides in the USA. HerbicidesDiquat

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HPLC Column Introduced to Simplify and Improve Separations of Herbicides Diquat and Paraquat