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GE Healthcare Launches Next Generation GenomiPhi™ Whole Genome Amplification Kits in Room Temperature Stable Ready-To-Go™ Format

Cytiva
25 Jul 2012

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GE Healthcare’s new illustra™ Ready-To-Go™ GenomiPhi™ kits provide researchers with a pre-dispensed, room temperature stable formulation for whole genome amplification, enabling a simplified workflow for obtaining large amounts of high quality DNA from small genomic DNA samples. The new kits also deliver improved yields over the current GenomiPhi kits.

GE Healthcare is the leader in DNA amplification based on the unique Phi29 DNA polymerase, employing the enzyme’s exceptional fidelity and processivity to prepare DNA for sequencing and genomic applications using the smallest of samples Previous GenomiPhi kits contain liquid enzyme formulations that require storage at -80°C degrees. The new kits incorporate GE Healthcare’s proprietary Ready-To-Go stabilisation technology which delivers single-dose reaction mixes in a solid format that can be stored for months at the bench without the need for refrigeration.

The new illustra Ready-To-Go GenomiPhi kits are available in two formats; Ready-To-Go GenomiPhi V3 improves upon the current GenomiPhi V2 kit with more than double the previous DNA yield, whilst Ready-To-Go GenomiPhi HY is specifically developed for high yield requirements, achieving 40 to 60 µg DNA yield from just 10 ng of starting DNA. Both kits are available in convenient 8-well strips or in 96-well plate formats.

illustra Ready-To-Go GenomiPhi kits can be used to amplify DNA from blood lysates and dried blood spots. A protocol has been developed for amplification from Whatman™ FTA card punches with minimal handling, thus enabling a streamlined, efficient workflow for sample collection and analysis.

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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Blood SpotDNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.DNA PolymeraseWhole Genome Amplification

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GE Healthcare Launches Next Generation GenomiPhi™ Whole Genome Amplification Kits in Room Temperature Stable Ready-To-Go™ Format