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Bruker Launches New High-definition Mass Spectrometer IMPACT HD™ for Trace Analysis of Complex Biological Samples

Bruker Daltonics
27 Jun 2013
Sarah Thomas
Associate Editor

Product news

Bruker recently introduced their exciting new High-Definition capabilities in proteomics and biomarker research at the 61st ASMS conference earlier this month. Patented low-noise Captivespray nanoBooster™ ionization coupled with record high-speed digitization Qq-TOF technology and steered by intelligent acquisition software defines complex samples more sharply than ever before.

The novel High-Definition accurate mass spectrometer, impact HD™ is the latest innovation in Bruker’s UHR-TOF product line. It opens up enhanced analytical performance levels for all applications where trace analysis from complex, high-background matrices is challenge – such as biomarker research, identification of impurities or residue screening. Thanks to extreme sensitivity broad mass-transfer ion optics and fastest ever 50Gbit/sec sampling technology a dynamic range of 5 orders of magnitude is achieved in one second of LC time. Wide dynamic range at high speed is a prerequisite for high-resolution detection of low-abundance compounds in sharp UHPLC peaks and permits direct quantitation without re-run.

The HD performance is directed by unique intelligent self-optimizing Instant Expertise™ software. MSMS acquisition parameters are dynamically adapted precisely to the incident sample, independent of sample amount or complexity, translating directly into higher productivity and expert-caliber results first time.

Bruker also introduces the CaptiveSpray nanoBooster™ which complements all Bruker LCMS instruments for new performance levels in proteomics and glycoanalysis. The new version of Bruker’s established CaptiveSpray source employs a novel dopant-enriched gas ionization environment to boost sensitivity, enhance charge state and reduce background noise to drive up protein ID rates significantly. The ability to engineer enhanced charge state is especially beneficial on Bruker’s amazon ETD ion trap in the structural analysis of glycopeptides by ETD.

The features ensure IMPACT HD delivers the fastest time-to-success for customers across a broad applications portfolio ranging from small molecule identification and characterization; food, forensic and environmental screening, metabolomics, proteomics to antibody analysis.

“Dynamic range really means something in your proteomics analysis when it can genuinely be delivered at UHPLC speeds” commented Carsten Baessmann Head of Applications Development “today we’re showing in a proteomics sample more than 8440 proteins at a false discovery rate (FDR) of better than 1% are identified from a combined 2D LC separation experiment with a CaptiveSpray nanoBooster™ - impact HD system.”

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :MetabolomicsMetabolomics is the study of small metabolites (the intermediates and products of metabolism). It involves the identification and quantification of cellular metabolites using analytical technologies such as GC, HPLC, NMR, and LC/MS.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Trace Level ImpuritiesTrace level impurities refer to low concentrations of unwanted substances found in chemicals, pharmaceuticals, and other products. Detecting and removing these impurities is crucial for product safety and regulatory compliance. Explore trace impurity analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Forensic InvestigationForensic investigation is a diverse collection of scientific techniques and methods for the identification and characterization of chemicals relating to crime scene investigations.Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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Bruker Launches New High-definition Mass Spectrometer IMPACT HD™ for Trace Analysis of Complex Biological Samples