SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Bio-Rad launches three StarBright dyes for flow cytometry

Bio-Rad

The StarBright Blue 700, StarBright Violet 440 and StarBright Violet 610 dyes aim to offer maximal brightness for improved resolution

3 Nov 2020
Diane Li
Assistant Editor

Product news

Bio-Rad Laboratories, Inc., a global leader of life science research and clinical diagnostic products, has launched three StarBright Dyes specifically developed for flow cytometry.

StarBright Blue 700, StarBright Violet 440, and StarBright Violet 610 are fluorescent nanoparticles that are conjugated to Bio-Rad’s highly validated flow antibodies, providing maximal brightness and improved resolution. Brighter than other fluorescent dyes with similar excitation and emission spectra, the StarBright dyes provide researchers with an alternative for flow cytometry panels. The brightness of the dyes combined with precise excitation and emission profiles offer improved resolution of rare populations and low-density antigens and easily integrate into multiplex panels.

“StarBright Dyes offer exceptional brightness with specific excitation and emission, allowing researchers to build brighter flow cytometry panels to enable better resolution of positive populations from background without the need for special buffers,” said Mike Blundell, Bio-Rad Product Manager, Life Science Group. “The unique profile of these dyes combined with Bio-Rad’s high-quality antibodies provides flexibility and offers a reliable alternative for incorporation into flow cytometry protocols.”

Key Benefits of StarBright Dyes

  • Resistant to photobleaching and highly stable with minimal lot-to-lot variation, enabling researchers to achieve consistent and reproducible staining
  • Work with all common staining buffers, including special polymer dye staining buffers, for easy integration into multicolor panels
  • The unique chemistry ensures no loss of signal in fixation
  • Offer narrow excitation and emission profiles for improved resolution in new and existing flow cytometry experiments

StarBright Dyes are compatible with Bio-Rad’s ZE5 Cell Analyzer, S3e Cell Sorter, as well as other conventional and spectral flow cytometers.

Do you use Bio-Rad products in your lab? Write a review today for your chance to win a $400 Amazon Gift Card>>

Related products

Request Quote for All Products

StarBright™ Blue 700 Fluorescent Secondary Antibodies

Bio-Rad

The StarBright Blue 700 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 470 nm and emission maximum at 705 nm. StarBright Blue 700 is a brighter alternative to PerCP-Cy5.5, with less spillover, and does not exhibit tandem breakage.

(0)

StarBright™ Blue 520 Fluorescent Secondary Antibodies

Bio-Rad

The StarBright Blue 520 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 488 nm and emission maximum at 520 nm. The StarBright Fluorophore is made up of multiple light-absorbing and -emitting monomers which provide an exceptionally bright signal compared to most traditional fluorophores.

(5)

Links

Bio-RadCompany website

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Clinical MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.Clinical StudyProspective biomedical or behavioral research studies on human subjects, following tests in animals, that are designed to answer specific questions about novel vaccines, drugs, treatments, dietary supplements, devices or new ways of using known interventions, generating safety and efficacy data.Molecular DiagnosticsMolecular diagnostics use an individual’s genetic code and gene expression to diagnose and monitor diseases. The technique is used increasingly in the field of infectious diseases and oncology, as well as areas such as coagulation, HLA typing and pharmacogenomics. Molecular diagnostics plays a pivotal role in personalized medicine.Dyes

Related articles

Bio-Rad launches three StarBright dyes for flow cytometry