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Application note: An optimized, multiplexed assay for screening ex vivo conditions which increase memory T cell frequency

Sartorius Group

Download this application note from Sartorius to discover how the need to monitor T cell phenotype and function for improved T cell expansion protocols was addressed with a T cell memory cell and cytokine profiling assay

25 Jun 2020
Sophie Ball
Publishing / Media

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Ex vivo expansion of T cells is a critical process in bio-manufacturing of adoptive cell therapies such as chimeric antigen receptor (CAR) T and tumor infiltrating lymphocyte (TIL) therapies.

Recent clinical studies suggest that ex vivo T cell expansion protocols generating higher percentages of T memory stem cells (Tscm), central memory T cells (Tcm) in the total cell product are critical to significant clinical improvements in adoptive cell therapies.

Download this application note to learn how the need to monitor T cell phenotype and function for improved T cell expansion protocols was addressed with a T Cell Memory Cell and Cytokine Profiling assay that enabled measuring memory cell phenotype, cell viability and effector cytokine release in the same sample well in a miniaturized assay format.

Download the application note paper here>>

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Application note: An optimized, multiplexed assay for screening ex vivo conditions which increase memory T cell frequency