All you need to know about HPLC columns in under an hour

Don't miss upcoming masterclass on how to maximize the lifetime and performance of your LC columns

17 Apr 2020
Tom Casburn
Associate Editor

Expert insights

Andreas Borowiak, Agilent Technologies
Andreas Borowiak, HPLC columns expert, Agilent Technologies

In this upcoming free webinar, HPLC columns expert Andreas Borowiak from Agilent Technologies will explain what you can do to achieve the maximum column lifetime and performance from your LC columns.

Are you looking for a comprehensive summary on how to protect your HPLC column from early failure, clogging and performance loss?

Are you tired of the endless entertainment options on Netflix and YouTube and looking for something with a bit more substance? Then register below to join this educational webinar.

Register Now


In this webinar, you will learn:

  • What a typical lifetime for an HPLC column should be
  • How to identify the root cause for early column failure (based on chromatograms)
  • How to protect your column from solvent contaminations, particles and sample matrix
  • How to select the right column based on your method needs (e.g., temperature, pH, pressure range)
  • How to properly clean and store your HPLC column

Who should attend?

  • LC operators who are new to the HPLC world
  • Scientists keen to refresh their knowledge of LC column troubleshooting
  • Instrument self-maintainers who want to improve their skillset
  • Everyone interested in new products that help maximize column lifetime

The live webinar and will take place on Wednesday, April 29, at:

  • 10:00 BST
  • 11:00 CEST

Register to watch the full webinar >>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars >>

Agilent 1290 Infinity II LC System

Agilent Technologies

The 1290 Infinity II LC System is an ultra-high performance LC that has market-leading sample capacity and is designed for the lowest dispersion for the most challenging ultra-HPLC experiments. The 1290 Infinity II LC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your existing laboratory environment.

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Agilent 1260 Infinity II Prime LC System

Agilent Technologies

The 1260 Infinity II Prime LC is a versatile HPLC offering the highest functionality and operational convenience for analytical HPLC and entry-level quaternary UHPLC at pressures up to 800 bar and for flow rates up to 5 mL/min. This versatile HPLC system is a perfect front end for your MS system. 

(29)

Agilent Pursuit C18 (USP L1) HPLC Columns

Agilent Technologies

200A pore size;  available in 2.0, 3.0, 3.9, 4.0, 4.6, 10 , 21.2 and 50  mm ids, and lengths from 50 - 250 mm. Pursuit columns are ideal for pharmaceutical applications using LC/MS, from analyzing lead compounds and biological samples in drug discovery, to implementing dependable analysis of raw materials and approved drugs in the QC process.  Build on the large 200A pore size silica, high liquid density delivers up to 40% faster separations without sacrificing resolution.

(68)

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.