A Rapid Method for Targeted and Untargeted Alkaloid Screening

23 Mar 2016
Lynsey Forsyth
Post Doc / Research Fellow

Editorial article

SelectScience® spoke to leading scientists at RAFA 2015 about their research, and the technology they are using. Watch the video to hear Dr Tiziana Nardin, Food Technologist at the Edmund Mach Foundation of San Michele, present her poster on analytical methods for alkaloid analysis.

Hear how targeted and non-targeted alkaloid methods enabled more than 120 different alkaloid-containing plants to be analyzed. A SPE on-line preparative system, coupled to UHPLC high-resolution mass spectrometry, analysis was optimized for fast and efficient identification of alkaloids.

Watch the Video

Download the Poster

Links

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.