Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: DNA Ligase I antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 102, 89, 99 kDa. Observed band size: 140 kDa.