Western Blot. Positive WB detected in Hela whole cell lysate,A549 whole cell lysate(treated with Calyculin A or EGF). All lanes Phospho-CDK1/CDK2/CDK3 antibody at 0.76μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34 KDa. Observed band size: 34 KDa.