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RAB27A Recombinant Monoclonal Antibody

Product Details

Cat. No.
CSB-RA285197A0HU
Type
Primary Antibody
Clonality
Monoclonal
0.0/5.0
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  • Overlay Peak curve showing PC3 cells stained with CSB-RA285197A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.

    Overlay Peak curve showing PC3 cells stained with CSB-RA285197A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.

  • Overlay Peak curve showing PC3 cells stained with CSB-RA285197A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.

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Applications

  • ELISA (ELISA)
  • Flow Cytometry (FC)

Antibody Overview