Western Blot. Positive WB detected in: 293 whole cell lysate(treated with Calyculin A or not). All lanes: Phospho-TP53 antibody at 0.64μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 KDa. Observed band size: 53 KDa