Western Blot. Positive WB detected in Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF). All lanes Phospho-GYS1 antibody at 1.12μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85 KDa. Observed band size: 85 KDa.