Western Blot. Positive WB detected in A549 whole cell lysate锛孒epG2 whole cell lysate(treated with EGF or Pervanadate). All lanes Phospho-EGFR antibody at 1.28μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 175 KDa. Observed band size: 175 KDa.