Western blot. All lanes: AP4B1 antibody at 3ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: U251 whole cell lysate. Lane 3: LO2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 84, 35 kDa. Observed band size: 84 kDa.