Western blot. All lanes: SPIN1 antibody at 1.45µg/ml. Lane 1: Hela whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 30 kDa. Observed band size: 30 kDa