Western blot. All lanes: LRRC42 antibody at 10ug/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: HCT116 whole cell lysate. Lane 4: Colo320 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa.