Western blot. All lanes: MMACHC antibody at 5.16µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: Raji whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa