Western blot. All lanes: MINPP1 antibody at 14ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: U251 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 56, 35, 32, 34 kDa. Observed band size: 56 kDa.