Western blot. All lanes: VPS4Aantibody at 4.28ug/ml. Lane 1: Mouse kidney tissue. Lane 2: Rat gonadal tissue. Lane 3: Mouse brain tissue. Lane 4: U87 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa.