Western blot. All lanes: COG5 antibody at 2.04 ug/ml. Lane 1: Mouse large intestine tissue. Lane 2: A549 whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 93, 95, 91 kDa. Observed band size: 93, 70 kDa.