Western blot. All lanes: VANGL2 antibody at 2.26ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 60 kDa. Observed band size: 60 kDa.