Western blot. All lanes: BAG family molecular chaperone regulator 5 antibody at 2ug/ml. Lane 1: Mouse kidney tissue. Lane 2: Mouse brain tissue. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 52, 57 kDa. Observed band size: 52 kDa.