Western blot. All lanes: TRHDE antibody at 1.77ug/ml. Lane 1: Rat heart tissue. Lane 2: Mouse kidney tissue. Lane 3: 293T whole cell lysate. Lane 4: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 117 kDa. Observed band size: 117 kDa.