Western blot. All lanes: TRIM15 antibody at 2µg/ml. Lane 1: Jurkat whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 53, 13 kDa. Observed band size: 53 kDa