Western blot. All lanes: VPS25 antibody at 3µg/ml. Lane 1: Mouse kidney tissue. Lane 2: HL60 whole cell lysate. Lane 3: THP-1 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa