Western blot. All lanes: Thioredoxin reductase 2, mitochondrial antibody at 6ug/ml. Lane 1: Mouse liver tissue. Lane 2: Mouse gonad tissue. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 57, 54, 47, 31 kDa. Observed band size: 57 kDa.