Western blot. All lanes: Histone-lysine N-methyltransferase SMYD3 antibody at 3ug/ml. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 50, 30, 43 kDa. Observed band size: 50 kDa.