Western blot. All lanes: SYT11 antibody at 14ug/ml. Lane 1: COLO205 whole cell lysate. Lane 2: Mouse skeletal muscle tissue. Lane 3: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.