Western blot. All lanes: FZD3 antibody at 1.94µg/ml. Lane 1: THP-1 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 77 kDa. Observed band size: 77 kDa