Western blot. All lanes: COG4 antibody at 4.85µg/ml. Lane 1: Mouse liver tissue. Lane 2: Mouse gonadal tissue. Lane 3: K562 whole cell lysate. Lane 4: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 90, 39, 82 kDa. Observed band size: 90 kDa