Western blot. All lanes: NIF3L1 antibody at 0.2ug/ml. Lane 1: 293T whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 42, 39, 33 kDa. Observed band size: 42 kDa.