Western blot. All lanes: UXT antibody at 3.2 ug/ml. Lane 1: HL60 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 18 kDa. Observed band size: 18, 80, 55 kDa.