Western blot. All lanes: Aladin antibody at 5ug/ml. Lane 1: Hela whole cell lysate. Lane 2: Mouse brain tissue. Lane 3: NIH/3T3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 60, 56 kDa. Observed band size: 60 kDa.