Western blot. All lanes: UBE2Z antibody at 1.68µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 39, 29 kDa. Observed band size: 39 kDa