Western blot. All lanes: UBA5 antibody at 6ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Caco-2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45, 39 kDa. Observed band size: 45 kDa.