Western blot. All lanes: SAR1A antibody at 4.57ug/ml. Lane 1: Mouse lung tissue. Lane 2: NIH/3T3 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 23, 18 kDa. Observed band size: 23 kDa.