Western blot. All lanes: NBPF3 antibody at 1.2µg/ml. Lane 1: PC-3 whole cell lysate. Lane 2: HT29 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 73, 68, 72, 33, 66 kDa. Observed band size: 73 kDa