Western blot. All lanes: POLR3C antibody at 2µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Mouse brain tissue. Lane 4: THP-1 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 61 kDa. Observed band size: 61 kDa