Western blot. All lanes: ARL8B antibody at 4µg/ml. Lane 1: Mouse brain tissue. Lane 2: NIH/3T3 whole cell lysate. Lane 3: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 22, 19 kDa. Observed band size: 22 kDa