Western blot. All lanes: GPR83 antibody at 2µg/ml. Lane 1: HCT116 whole cell lysate. Lane 2: U87 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa