Western blot. All lanes: IL21R antibody at 2.18ug/ml. Lane 1: A549 whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: Colo320 whole cell lysate. Lane 4: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa.