Western Blot. All lanes: TTC12 antibody at 8µg/ml. Lane 1: Mouse large intestine tissue. Lane 2: Mouse small intestine tissue. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 79, 82 kDa. Observed band size: 79, 170 kDa