Western blot. All lanes: SDF4 antibody at 6µg/ml. Lane 1: U87 whole cell lysate. Lane 2: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 42, 31, 28, 24, 16, 40 kDa. Observed band size: 42 kDa