Western blot. All lanes: Methylosome protein 50 antibody at 6ug/ml. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 37, 30 kDa. Observed band size: 37 kDa.