Western blot. All lanes: ACVR1C antibody at 4ug/ml. Lane 1: Mouse brain tissue. Lane 2: HepG2 whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 55, 38, 47, 50 kDa. Observed band size: 55 kDa.