Western blot. All lanes: Histone-lysine N-methyltransferase EZH1 antibody at 4ug/ml. Lane 1: Hela whole cell lysate. Lane 2: HL60 whole cell lysate. Lane 3: Mouse gonad tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 86, 87, 81, 77, 69 kDa. Observed band size: 86 kDa.