Western blot. All lanes: PIK3R3 antibody at 3.5ug/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Mouse gonadal tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 55, 48, 44 kDa. Observed band size: 55 kDa.