Western Blot. All lanes: GCDH antibody at 8µg/ml. Lane 1: Mouse kidney tissue. Lane 2: Mouse liver tissue. Lane 3: Hela whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: LO2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49, 48 kDa. Observed band size: 49 kDa